Question

1. Which of the following is/are required for the polymerization reaction catalyzed by RNA polymerase?

1) RNA primer

2) DNA primer

3) RNA template

4) DNA template

5) NTPs

6) dNTPs

7) Magnesium

8) Calcium

A) 4, 5, and 6

B) 1, 3, 5, and 7

C) 1, 3, 5, and 8

D) 4, 5, and 7

2. RNA polymerase is best described as a ________.

A) DNA-dependent DNA polymerase

B) RNA-dependent DNA polymerase

C) RNA-dependent RNA polymerase

D) DNA-dependent RNA polymerase

3. Eukaryotic RNA polymerase II requires which proteins for selective binding?

A) ? subunit

B) ? subunit

C) Transcription factors

D) ? subunit

4. A deficiency in intracellular rRNA can be attributed to which eukaryotic enzyme?

A) RNA polymerase II

B) DNA polmerase I

C) RNA polymerase III

D) RNA polymerase I

Answer 1

Ans-1- RNA polymerase catalyzes the transcription reaction which makes RNA and requires NTPs, DNA template and Mg²⁺ hence option D is right.

Ans-2- as RNA polymerase uses DNA as a template to make RNA it is dependent on DNA for transcription. Hence RNA polymerase is DNA dependent RNA polymerase.

Ans-3- eukaryotic RNA polymerase requires multiple transcriptio factor (TFIID, TFIIA, TFIIH, etc) and TATA binding protein in order to bind promoter.

Ans-4- RNA polymerase l catalyzes the the synthesis of rRNA and its deficiency leads the cells to suffer from scarcity of rRNA. Hence option D is correct.