Question

In the reduction of CDP to dCDP in E. coli cells, what group is abstracted by...

In the reduction of CDP to dCDP in E. coli cells, what group is abstracted by the thiyl radical on the enzyme? Please be very specific about the nature of the group and where it is located on the substrate.

(b) For the same reaction, what is the precise role of the flavin cofactor? A good answer will be one long sentence or two shorter sentences.

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Answer #1

3'H of substrate is abstracted by the thiyl radical of Cys439 of enzyme subunit R2.

Flavin cofactor reduced ferrous iron in metR2 subunit to ferric iron and tyrosyl radical generated by oxygen in reduced R2 subunit.

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