Question

How does TAE or TBE buffer increase conductivity during agarose gel electrophoresis?

How does TAE or TBE buffer increase conductivity during agarose gel electrophoresis?

Homework Answers

Answer #1

Tris-Borate-EDTA and Tris-Acetate-EDTA both are most common type of buffer used in agarose gel electrophoresis of DNA. Firstly, EDTA is used as an chelating agent which chelates metal ion. Metal ion required as a co-factor for nuclease (DNAse or RNAse). Hence EDTA as a part of buffer prevent sample from nuclease activity. The most important function of TAE/ TBE buffer is to provide electric conductivity. Water molecules electrolyze into H+ and OH- at high current. H+ may binds to negatively charged DNA molecules and cannot migrate in gel. Buffer maintain the constant pH around neutral which prevent electrolysis of water molecules and help DNA to migrate towards positive electrode.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Recall that you used agarose powder, 1X TBE buffer, and a fluorescent dye, GreenGlo, to make...
Recall that you used agarose powder, 1X TBE buffer, and a fluorescent dye, GreenGlo, to make an agarose gel in lab. Calculate the amounts for following reagents to make a 2.0% - 50 ml agarose gel. Answer the amounts for GreenGlo in microliters and the amounts for TBE buffer and diluent in milliliters. (3 points) a. Agarose powder (g) (1 point) b. 20,000X GreenGlo (μl) (1 point) c. 10X TBE Buffer. Include the amount of diluent. (ml) (1 point)
1X TAE buffer is commonly used as a buffer for DNA gel electrophoresis. You will preform...
1X TAE buffer is commonly used as a buffer for DNA gel electrophoresis. You will preform the calculations necessary to prepare a 50X concentrated stock to store and use throughout the semester. The composition of a 50X TAE stock buffer is 2 M Tris, 1 M acetic acid, 50 mM EDTA, pH 8. Determine how much of each component you will need to make 100 ml of a 50X stock buffer. Show your calculations and construct a table which contains...
We cannot observe the DNA with naked eye during run on agarose gel electrophoresis since DNA...
We cannot observe the DNA with naked eye during run on agarose gel electrophoresis since DNA is colorless and very low amount of DNA is used in this technique. a) Considering the charge of DNA, what is the direction of DNA migration when it is run on agarose gel electrophoresis? (5 pts) b) How do we observe the DNA on agarose gel electrophoresis if DNA is colorless? (5 pts) c) If you want to run an oligonucleotide (25 bp) on...
Which way does the dna migrate on a agarose gel electrophoresis and why?
Which way does the dna migrate on a agarose gel electrophoresis and why?
What amount of agarose (g) and TAE buffer (mL) would you need to make a 1%...
What amount of agarose (g) and TAE buffer (mL) would you need to make a 1% gel?
.           Describe in detail, how you would prepare a 0.8% agarose gel, which is 1X TAE...
.           Describe in detail, how you would prepare a 0.8% agarose gel, which is 1X TAE and 0.1ug/ml Ethidium bromide. Your gel box holds 50 ml. TAE is 50X, Ethidum bromide is 10 mg/ml.
Which of the following sequences would run the slowest through an agarose gel during gel electrophoresis?...
Which of the following sequences would run the slowest through an agarose gel during gel electrophoresis? a) 5'- AT -3' b) 5’- ATGCTGC -3’ c) 5’- ATGCTGCAGTTA -3' d) All of these sequences would run at the same speed
What is the purpose of ethidium bromide in agarose gel electrophoresis?
What is the purpose of ethidium bromide in agarose gel electrophoresis?
What is the purpose of agarose gel electrophoresis and why is it used?
What is the purpose of agarose gel electrophoresis and why is it used?
A)Why do nucleic acids move towards the positive electrode in agarose gel electrophoresis at pH8? B)....
A)Why do nucleic acids move towards the positive electrode in agarose gel electrophoresis at pH8? B). In general, when analyzing DNA in agarose gel, DNA size marker is also applied to one of the wells. In this experiment, it doesn’t require to load DNA size marker when analyze your isolated plasmid by agarose gel electrophoresis, why?