Question

How does TAE or TBE buffer increase conductivity during agarose gel electrophoresis?

How does TAE or TBE buffer increase conductivity during agarose gel electrophoresis?

Homework Answers

Answer #1

Tris-Borate-EDTA and Tris-Acetate-EDTA both are most common type of buffer used in agarose gel electrophoresis of DNA. Firstly, EDTA is used as an chelating agent which chelates metal ion. Metal ion required as a co-factor for nuclease (DNAse or RNAse). Hence EDTA as a part of buffer prevent sample from nuclease activity. The most important function of TAE/ TBE buffer is to provide electric conductivity. Water molecules electrolyze into H+ and OH- at high current. H+ may binds to negatively charged DNA molecules and cannot migrate in gel. Buffer maintain the constant pH around neutral which prevent electrolysis of water molecules and help DNA to migrate towards positive electrode.

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