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what does mean if you have a distribution recovered activity for homogenate 102% and for mitochondria...

what does mean if you have a distribution recovered activity for homogenate 102% and for mitochondria 0.2%?

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Answer #1

This is basically the measurement of enzyme activity in homogenate vs mitochondrial fractions.

The assay is performed in fractions of homogenate and mitochondrial fractions. The activity of enzymes is more in homogenate (102%) compared to activity in mitochondrial fraction (0.2%).

Homogenate is a complete cell suspension of homogenized tissue which contain all the contents of cytoplasm and organelles.

Mitochondrial fraction contains only mitochondrial proteins.

The activity of enzyme described above is more in homogenate than mitochondria indicating that enzyme is a not-mitochondrial enzyme.

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