Question

Why are supernatant and pellet generated during centrifugation?

Why are supernatant and pellet generated during centrifugation?

Homework Answers

Answer #1

During centrifugation, centrifugal force is applied & supernatant & pellet are separated due to principle of sedimentation. Due to the application of centrifugal force, substances with higher density move outward in the radial direction & substances with higher density move to the center. Pellet is the concentrated substances & due to their high density compared to the supernatant (Liquid phase) it comes to the bottom & supernatant comes to the top. As a result, we get two different phases.

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