Question

19.    SDS-PAGE and the isoelectric focusing method for the separation of proteins both have which of the following characteristics in common?

            (i)        Separate native proteins.

            (ii)       Make use of an electric field.

            (iii)      Separate proteins according to their mass.

            (iv)      Require a pH gradient.

(v) Are carried out on supporting gel matrices.

Answer 1

Solution

Option (ii) Make use of an electric field and Option (v) Are carried out on supporting gel matrices are true

Both SDS-PAGE and the isoelectric focusing method for the separation of proteins make use of an electric field and are carried out on supporting gel matrices​​​​​​​

SDS page is used to separate denatured protein. Therefore option (i) is wrong

Only SDS-PAGE estimate relative molecular mass of protein, separate proteins according to the charge of proteins. therefore option (iii) is wrong

SDS-PAGE do not require a pH gradient. Therefore option (iv) is wrong