Question

. The value of the firefly luciferase gene in cloning could best described as

A) the gene provides valuable restriction enzyme cut sites.

B) the gene “reports” if a recombinant product is formed.

C) the gene confers resistance to antibiotics.

D) the gene facilitates easier transfer of the DNA into bacterial cells.

            E) the gene “reports” that a cloned DNA contains a promoter or silencers or enhancers.

Answer 1

Luciferase gene doesn't provide any valuable restriction enzyme cut sites.

Luciferase gene doesn't provide any antibiotic resistance to the transformed cell. So option C is wrong.

Luciferase doesn't express by itself it needs to be induced by some condition.

Luciferase gene doesn't facilitate the easier transfer of the DNA into bacterial cells. The transfer is facilitated by heat shock.

Luciferase gene is cloned under a promoter or silencers or enhancers sequence whose activity we need to check at the given experimental condition. If it is expressed then we need to check its activity by performing luciferase assy.

So, otpion E is the correct answer.