Question

An E. coli strain of chromosomal genotype lacI+ lacP- lacO+ lacZ+ lacY+. You wish to transform...

An E. coli strain of chromosomal genotype lacI+ lacP- lacO+ lacZ+ lacY+. You wish to transform this strain into a wild-type lac operon by the addition of an extra piece of DNA (plasmid). a. What gene or genes would you add on this extra DNA that would make this strain express the lac operon genes as wild type? ?b. How would you design thus plasmid if you want the strain of E. coli to express the lac operon genes even if glucose in present in the medium?

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