Question

Which of the following are assays which detect the ACTIVITY or FUNCTION of a protein enzyme?...

Which of the following are assays which detect the ACTIVITY or FUNCTION of a protein enzyme? You may choose more than one answer.

Western Blot

ELISA

Hormone-stimulated adenylyl cyclase activity assay to detect the production of cAMP (using ELISA to detect cAMP)

Southern Blot

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Answer #1

The right answer is option c or " Hormone-stimulated adenylyl cyclase activity assay to detect the production of cAMP (using ELISA to detect cAMP) ".

The adenylyl cyclase activity assay is used to detect the actuvity of Adenylyl cyclase activity . The adenylyl cyclase is a protein enzyme help to produce cAMP from ATP.

Southern Blot is used to detect the DNA it is not involved any protein realated activity.

Western Blot is used to detect the specific protein or length of the protein not the enzyme.

ELISA is used to detect antigen and antibody present in the blood. it works like enzyme substrate activity but not detect any protein enzyme.

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