Question

Which of the following are assays which detect the presence of a protein enzyme? You may...

Which of the following are assays which detect the presence of a protein enzyme? You may choose more than one answer.

Western Blot

Southern Blot

ELISA

Specific Binding Assay

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Answer #1

Answer: Western blot, ELISA and specific binding assay.

Explanation:

Western blotting is an analytical technique used for the detection of proteins in the sample.

ELISA stands for enzyme-linked immunosorbent assay and it is a plate-based method for the detection and quantification of proteins, antibodies etc.

Specific binding assay is based on the interaction between a protein and its ligand. This assay is commonly used for determining the binding affinity of enzymes with its ligands. But this technique can be used for the detection of enzymes using its ligands which are radiolabeled.

Southern blotting is a technique used for detection of specific DNA sequence in the sample.  

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