Question

Illustrate a gel, with bands representing the RFLP's for each of the individuals below. Be sure...

  • Illustrate a gel, with bands representing the RFLP's for each of the individuals below. Be sure to include the length of each fragment, and positive and negative nodes.
  • Restriction enzyme information:
    • Binds to ATTCC
    • Cuts between A-T
  • Individual 1: ATTCCATTCC
  • Individual 2: AATTCCAATTCC
  • Individual 3: ATCCATTCC
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Answer #1

The Sequences of Individuals are  

Individual 1: ATTCCATTCC

Individual 2: AATTCCAATTCC

Individual 3: ATCCATTCC

The restriction digestion of the DNA sequence of individual 1

A TTCCA TTCC

3 bands will be formed: A (one nucleotide) TTCCA (5 Nucleotides) TTCC (4 nucleotides)

The restriction digestion of the DNA sequence of individual 2

  AA TTCCAA TTCC

3 bands will be formed: AA ( 2 nucleotides) TTCCAA (6 Nucleotides) TTCC (4 nucleotides)

The restriction digestion of the DNA sequence of individual 3

ATCCA TTCC

2 bands will be formed: ATCCA ( 5 nucleotides) TTCC (4 nucleotides)

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