Question

Describe the synthesis of DNA polymerase starting with the synthesis of the mRNA (transcription has already happened). Stop here and think about the protein DNA polymerase – what does it do? where does it function? (you should know this from general biology – if not, look it up!)

Explain the details of how this protein is made and targeted to its final destination. Since you are starting with mRNA, be sure to discuss how the mRNA gets to the ribosome.

This question is not about DNA polymerase activity – this chapter is about protein synthesis, so you are applying this knowledge to the protein DNA polymerase!

Answer 1

Subunits of Ribosomes are found dissociated in cytoplasm and when mRNA is present then smaller subunit is bound at mRNA. mRNA posses ribosome binding site which is a consensus sequence called shine dalgnow sequence and then ionic changes occur and larger subunut bind at smaller subunit and complimentry tRNA synthesise the peptide chain on p site. Then at stop codon syntesis is over and peptide chain is released into ER lumen. From there it is transferred to golgi complex and Golgi body process and a vasicle containing protein is released and signal sequence guides the protein to functional site. At functional site chaperones are released and protein folded to perform ther function.