An isolated bacterial colony is a group of bacteria that has grown from a single organism. It has been grown in certain conditions that are conducive for its growth on certain growth media. These kinds of conditions are responsible to separate it from other bacterial species by preventing their growth. When we streak for isolated colonies, we dilute our inoculum by streaking it across the agar. Our intension is to get the inoculums diluted to the point where there is only one bacterial cell deposited every few millimetres on the agar plate surface. These lone bacterial cells divide and produce thousands of new bacterial cells, and we get an isolated colony.
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