Question

Use your knowledge of how DNA polymerase works and the structure of DNA to explain why continuous synthesis of both DNA strands is not possible (12.1)

Answer 1

Answere : DNA replication always occurs in 5' to 3' direction.because DNA polymerase require free 3' OH group for addition of new nucleotide to primer. Replicationoccur on both strand. Replication occur in continuous manner on 3'-5' parental strand and the new strand is called leading strand. Replication occur in discontinuous manner on 5'-3' parental strand and new strand is calles lagging strand. Lagging strand require more than one primers for new strand synthesis. This result in the okazaki fragement which later joined by DNA ligase.