Question

Question Use the UCSC genome browser to find out how many exons are in the human
gene MESP1 ?
Answer 2

Which of the following is complementary strand of DNA to the following
sequence.
5?ACTCGGTAA 3?
Answer
3?TGAGCCATT 5?

I know the answers. What I am asking is how can you tell me in detail how to solve it please its for bioinformatics.

Answer 1

for the number of exons of the gene MESP1, we need to go tho the UCSC genome browser home page. There under the genome browser category, we can search of desired human genes. When we search for MESP1 gene, all the information related to MESP1 will be shown and there we can see that the gene has 2 exons.

As adenine (A) will always pair with Thymine (T) and Guanine (G) will always pair with cytosine (C), we can write the complementary strand of DNA which will run in 3'-5' direction. When the given sequence is 5?ACTCGGTAA 3?, we only require to replace the A with T and G with C in the complementary strand and it will run in the direction 3' to 5'

thus the sequcence complementary to 5?ACTCGGTAA 3? will be 3' TGAGCCATT