Question

Indicate which of the following is least important in doing a complementation test with coinfection of phage T4.

		recovering phage from the plaques after growth and lysis
		ensuring that sufficient phage of both strains are present
		counting the plaques that are produced on E. coli K(?)
		control using both mutations in cis configuration and a wild type

Transcription and translation can be coupled in bacteria but not eukaryotes since

		there is no nuclear membrane in eukaryotes.
		there is no nuclear membrane in prokaryotes.
		the bacterial DNA is in the nucleoid
		eukaryotic chromosomes are found in nucleoids.

Answer 1

Indicate which of the following is least important in doing a complementation test with coinfection of phage T4.
The answer is recovering phage from the plaques after growth and lysis

Transcription and translation can be coupled in bacteria but not eukaryotes since
The answer is there is no nuclear membrane in prokaryotes.

The main reasons are...
The presence of nuclear membrane,
post transcriptional modifications,
the presence of ribosomes in cytoplasam...etc.