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Suppose you have a mutant that is unable to grow on Min+glucose. What (genetic) reason other...

  1. Suppose you have a mutant that is unable to grow on Min+glucose. What (genetic) reason other than “cannot catabolize glucose” can explain such a result?

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Answer: Most bacterial species are auxotrophs. If a mutant is unable to grow in a medium containing glucose, then most probable reason is, the mutation in the auxotroph gene. In most of the cases, thiamine deficiency doesn't allow the growth to take place, so thiamine is additionally added. Second reason could be, in mutants, glucose may act as a catabolite repressor in some major pathways. In place of glucose, if sugars like lactose, arabinose or galactose is used with constant shaking then the growth could be observed.

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