Question

Part F - Recombination and resistance The introductory passage explains that Vibrio cholerae bacteria may benefit from being able to obtain genes from their prey. What would be the best way to test whether they can benefit within one week of exposure to prey with a gene that confers resistance to ampicillin, an antibiotic that is commonly used in microbiology laboratories? Choose all of the steps that you should include.

Transfer V. cholerae that have been exposed for one week to prey lacking the resistance gene to medium with ampicillin.

Set up V. cholerae cultures that are resistant to ampicillin and allow them to grow for a week in the presence of prey with the resistance gene on medium that does not contain ampicillin.

Set up V. cholerae cultures that are susceptible to ampicillin and allow them to grow for a week in the presence of prey lacking the resistance gene on medium that contains ampicillin.

Transfer V. cholerae that have been exposed for one week to prey with the resistance gene to medium with ampicillin.

Set up V. cholerae cultures that are susceptible to ampicillin and allow them to grow for a week in the presence of prey that have the resistance gene on medium that does not contain ampicillin.

Set up V. cholerae cultures that are susceptible to ampicillin and allow them to grow for a week in the presence of prey lacking the resistance gene on medium that does not contain ampicillin.

Set up V. cholerae cultures that are susceptible to ampicillin and allow them to grow for a week in the presence of prey that have the resistance gene on medium that contains ampicillin.

Set up V. cholerae cultures that are resistant to ampicillin and allow them to grow for a week in the presence of prey lacking the resistance gene on medium that does not contain ampicillin.

Answer 1

Set up V. cholerae cultures that are susceptible to ampicillin and allow them to grow for a week in the presence of prey that have the resistance gene on medium that does not contain ampicillin. ( this time the bacteria will take up the ampicillin gene)

Transfer V.Cholerae that have been exposed for one week to prey with resistance gene to medium with ampicillin. (The bacteris which will contain the ampicillin resistance gene will only be able to survive not others)