Question

Select statements from the list of options provided that are TRUE for the sequence provided below....

Select statements from the list of options provided that are TRUE for the sequence provided below. ATGGCATCCACACACCAATCATCCACAGAACCCTCTTCCACAGGTAAATCTGAGGAAACGAAGAAAGATGCTTCGCAAGGGAGCGGGCAAGACTCCAAGAACGTAACCGTTACCAAAGGTACCGGTTCCTCCGCCACCTCAGCTGCCATTGTCAAGACAGGAGGATCCCAAGGCAAAGATTCCTCTACTACAGCGGGCTCTTCTAGTACTCAGGGACAGAAGTTCAGTACTACACCTACCGACCCGAAAACTTTCAGCTCTGACCAAAAGGAGAAATCCAAAAGCCCAGCCAAAGAAGTCCCGTCTGGTGGCGATAGTAAGTCCCAAGGTGACACCAAGTCTCAAAGCGACGCCAAATCTTCTGGACAAAGTCAGGGCCAGTCTAAAGACAGCGGCAAATCATCTTCCGACAGTAGCAAGAGTCACTCTGTCATCGGAGCTGTCAAAGACGTCGTTGCAGGCGCCAAAGATGTCGCAGGAAAAGCCGTCGAGGATGCTCCTAGCATCATGCATACTGCAGTCGATGCTGTGAAGAACGCAGCCACGACTGTGAAGGATGTGGCATCGTCGGCTGCATCGACTGTGGCGGAGAAGGTAGTCGATGCTTACCACAGTGTGGTGGGAGACAAGACGGACGACAAGAAAGAGGGCGAGCACAGCGGCGACAAGAAGGACGACTCCAAAGCTGGAAGTGGCTCTGGACAAGGTGGTGACAACAAGAAGTCTGAAGGAGAGACTTCTGGCCAAGCAGAATCCAGCTCTGGCAACGAAGGAGCTGCTCCAGCCAAAGGCCGTGGTCGTGGACGGCCTCCAGCAGCTGCTAAAGGAGTTGCTAAGGGTGCTGCAAAGGGCGCTGCCGCCTCCAAAGGAGCCAAGAGCGGTGCTGAATCCTCCAAGGGAGGAGAACAGTCGTCAGGAGATATCGAGATGGCAGATGCTTCCTCCAAGGGAGGCTCGGACCAGAGGGATTCCGCGGCGACCGTTGGCGAAGGTGGTGCATCAGGCAGTGAGGGTGGAGCTAAGAAAGGCAGAGGGCGGGGCGCTGGTAAGAAAGCGGATGCGGGTGATACGTCCGCTGAGCCGCCTCGGCGGTCGTCCCGCCTGACGTCTTCAGGTACAGGGGCGGGTTCCGCTCCAGCTGCAGCGAAAGGCGGAGCGAAGCGTGCTGCTTCTTCCTCCAGTACACCTTCCAACGCTAAGAAGCAAGCGACTGGAGGTGCTGGCAAAGCTGCTGCCACCAAAGCAACTGCTGCCAAATCGGCAGCCTCTAAAGCTCCCCAGAATGGCGCAGGTGCCAAGAAGAAGGGAGGAAAGGCTGGAGGACGGAAGAGGAAGTAA *

  • The best approach to analyse this sequence is by using blastn only because the sequence provided is a DNA sequence.
  • The sequence comes from an organism that can survive radiation and the vacuum of space.
  • The are currently no other sequence homologs in the GenBank database.
  • A blastp search yielded the best number of homologous sequences.
  • There are two structures of this protein in the Protein Data Bank.

Homework Answers

Answer #1

The true statements are :

  • The best approach to analyse this sequence is by using blastn only because the sequence provided is a DNA sequence.
  • The sequence comes from an organism that can survive radiation and the vacuum of space.
  • The are currently no other sequence homologs in the GenBank database.

Explanation: As the sequence is an nucleotide sequence, the best method to analyse and find out homologous sequence is BLASTn where query sequence is analyzed with all the submitted nucleotide sequences available. The sequence analysis showed that it is the sequence of Ramazzottius varieornatus Dsup mRNA for damage suppressor, complete cds. This Ramazzottius varieornatus is a member of tardigrades which are usually resistant to extreme conditions. This organisms can survive exposure to the vacuum and radiation even in outer space.

When nucleotide sequence was tranlated to amino acid sequence and analyzed by blastp tool it also yiels one sequence only and that is : RecName: Full=Damage suppressor protein [Ramazzottius varieornatus]. So BLASTp do not yield the best number of homologous sequences and structures are not available in PDB. So first three statements are correct and last two statements are incorrect.

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