Question

Why does the gel need to be attached to a power supply? How does this make...

Why does the gel need to be attached to a power supply? How does this make the DNA fragments move?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Agarose gel electrophoresis is used to separate DNA fragments based on their size. DNA molecules are inherently negetively charged due to the presence of phosphate groups in its backbone. As a result, it migrates towards the positive electrode (Anode) under the influence of electricity. The voltage difference between two electrodes allows charged particles to move towards oppositely charged poles. The migration of different DNA molecules under the influence of electricity depends upon their size.


The migration distance of a DNA molecule in a gel is inversely proportional to its size. i.e. larger DNA molecules move slowly as compared to smaller molecules.
= Under the influence of electricity, smaller DNA fragments will be closer to the anode as compared to larger molecules.

Polyacrylamide gel electrophoresis is used to separate DNA fragments based on their charge or size.


0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
In which direction does the DNA run on the gel? Toward the positive or negative pole?...
In which direction does the DNA run on the gel? Toward the positive or negative pole? Why? 2. If you plug the read lead into the (-) source and the black lead into the (+) source on the power supply, what will happen to your DNA when you run your gel? .3. What is the purpose of the loading dye?
Which way does the dna migrate on a agarose gel electrophoresis and why?
Which way does the dna migrate on a agarose gel electrophoresis and why?
A)Why do nucleic acids move towards the positive electrode in agarose gel electrophoresis at pH8? B)....
A)Why do nucleic acids move towards the positive electrode in agarose gel electrophoresis at pH8? B). In general, when analyzing DNA in agarose gel, DNA size marker is also applied to one of the wells. In this experiment, it doesn’t require to load DNA size marker when analyze your isolated plasmid by agarose gel electrophoresis, why?
You’ve been asked to prepare the gel. How much agarose would you need to weigh in...
You’ve been asked to prepare the gel. How much agarose would you need to weigh in order to prepare 50 ml of 2% gel? Show your math. List three things to which one needs to pay special attention when loading a gel for DNA electrophoresis. What would be the volume of the PCR sample you would have loaded in live lab? The loading buffer has a dual purpose. Explain. There are multiple reasons for loading a DNA ladder (size standard)...
Why does the Money supply need to be controlled?
Why does the Money supply need to be controlled?
Why does the Money supply need to be controlled?
Why does the Money supply need to be controlled?
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection...
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is the _____ blot. Select one: a. Northern b. Western c. Northeastern d. Southern e. Southwestern 2. DNA sequencing by controlled termination of replication is called the _____ method. Select one: a. Sanger b. E. coli c. Restriction enzymes d. DNA Microarray e. Ligase f. Polymerase Chain Reaction g. Footprint h. Reverse Trancriptase i. Vector j. Expression k. Fluorescent l. cDNA 3. How...
complete the sentences with the appropriate term or phrase and arrange the sentences in order from...
complete the sentences with the appropriate term or phrase and arrange the sentences in order from what happen first to last. Pour molten agarose into an acrylic plate to which a comb is attached. Place the hardened gel into a tank with buffered solution and remove the comb. Load DNA samples mixed with a blue dye into the wells. Attach the positive and negative electrodes to a power supply and turn on the current. Negatively charged DNA molecules will move...
How could the accuracy and precision of the DNA size estimation using standard gel electrophoresis with...
How could the accuracy and precision of the DNA size estimation using standard gel electrophoresis with Agarose gel be improved? Give 3 ways! P.s. Not too technical perhaps considering it's my first year of university question What I mean is just 3 simple ways to make estimating size of DNA theough gel electrophoresis more accurate or more precise than just manually measuring the distance of DNA bands and comparing it to a reference sample DNA
How would you expect the lanes on a gel to differ if you cut the same...
How would you expect the lanes on a gel to differ if you cut the same DNA with each type of enzyme and why? (3)
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT