Question

Drag and drop the (+) and (-) symbols depending on if active ?-galactosidase is produced (+)...

Drag and drop the (+) and (-) symbols depending on if active ?-galactosidase is produced (+) or if if active ?-galactosidase is not produced (-) in the presence of lactose: lacI+ lacP+ lacOc lacZ– lacY+ blank lacI+ lacP+ lacO+ lacZ+ lacY+ blank lacIs lacP+ lacOc lacZ+ lacY+ blank lacI– lacP+ lacO+ lacZ+ lacY+ / lacI+ blank lacIs lacP+ lacO+ lacZ+ lacY+ / lacI+ blank

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Answer #1

lacI+ lacP+ lacOc lacZ– lacY+ - No galactosidase production

LacZ gene is mutated so no production

lacI+ lacP+ lacO+ lacZ+ lacY+ - No galactosidase production

LacI will inhibit mRNA synthesis

lacIs lacP+ lacOc lacZ+ lacY+ - LacIs will not be able to bind to operator as operator site is mutated.

Galactosidase production

lacI– lacP+ lacO+ lacZ+ lacY+ / lacI+ -LacI from the other plamsid iwll binds to the operator so  No galactosidase production

lacIs lacP+ lacO+ lacZ+ lacY+ / lacI+ -  No galactosidase production

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