Question

In the bacterial infection through bacteriophage lambda infection experiment, what is the specific function of molten...

In the bacterial infection through bacteriophage lambda infection experiment, what is the specific function of molten agar? Explain in detail.

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Bacteriophage lambda infect host cells by binding to maltose receptor expressed by the bacteria. Agar contains maltose that allows growth of the bacteria and they express the receptors necessary for infection by bacteriophage. Molten agar used in the assay allows bacteria (host) to be immobilized where they keep growing and provides an opportunity to the bacteriophages that are being released by the cells to diffuse short distancs in the culture plates, favouring infection of newer host cells. Lysis of the host cells during bacteriophage release leads to formation of clear regions called as plaques.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
In the late stages of infection of a bacterial cell by the bacteriophage T4, transcription of...
In the late stages of infection of a bacterial cell by the bacteriophage T4, transcription of cellular genes stops because the bacterial core RNA polymerases are redirected to a set of "late" genes in the T4 genome, which are then transcribed at a high level. This transition depends on production of the bacteriophage proteins gp33 and gp55, which are similar to bacterial sigma proteins. What feature or property of the T4 late genes could enable this transition? A. a different...
A bacteriophage is a virus that infects bacteria. In an experiment, if too few bacteriophages are...
A bacteriophage is a virus that infects bacteria. In an experiment, if too few bacteriophages are used for the infection, it may be difficult to detect or measure the response being tested. Therefore, it is important to determine the multiplicity of infection (MOI). The MOI is the ratio between the number of bacteriophages and the number of bacteria (number of bacteriophages/ number of bacteria). 1) A 0.1 ml aliquot of a bacteriophage stock with a concentration of 4 x 109...
Choose a bacterial, viral or parasitic infection and explain what you would look for on a...
Choose a bacterial, viral or parasitic infection and explain what you would look for on a CBC (complete blood count) to verify your suspicions.
In an experiment, E.coli cells have been genetically modified through site directed mutagenesis. This is a...
In an experiment, E.coli cells have been genetically modified through site directed mutagenesis. This is a process through which specific locations in an organism’s genome can be altered in order to study the function of such locations, or proteins coded for by genes found in a location. There are many techniques available for site directed mutagenesis. (You have access to PCR ingredients, any primer you wish, laboratory equipment, Lambda phage, and the ability to alter Lambda genome, a commercially available...
The genome of the bacteriophage M13 was modified to include a CRISPR-Cas 9 operon with the...
The genome of the bacteriophage M13 was modified to include a CRISPR-Cas 9 operon with the following features: The operon includes a gene coding for protein Cas 9, and a gene coding a sgRNA. The CRISPR-Ca9 operon is under the control of a bacterial constitutive promoter. The sgRNA gene includes a spacer flanked by two repeats. The sequence of the spacer matches a sequence of an antibiotic resistance gene present in an antibiotic resistant form of a bacteria. Bacteria containing...
In a spectrophotometry experiment, a wavelength near lambda maximum is chosen. What was the advantage of...
In a spectrophotometry experiment, a wavelength near lambda maximum is chosen. What was the advantage of using a wavelength near max lambda as opposed to using a wavelength where the absorbance is low?
“Experiment 2- Fluid Thioglycollate Medium to Assess the Effect of Oxygen on Bacterial Growth” “Table 2:...
“Experiment 2- Fluid Thioglycollate Medium to Assess the Effect of Oxygen on Bacterial Growth” “Table 2: Experiment 2 Results” “Sample Location” “Growth Location in FTM” “Oxygen Category” “Control” No growth N/A “Skin” Below surface growth and throughout agar None “Nose” Slight surface growth Facultative anaerobe “Throat” No growth Strict anaerobe “Shoe” Below surface growth and throughout agar Microaerophile “Post-Lab Questions” “3. Provide an explanation as to why bacteria from each location displayed its pattern of growth?” Click here to enter...
In an experiment, E.coli cells have been genetically modified through site directed mutagenesis. This is a...
In an experiment, E.coli cells have been genetically modified through site directed mutagenesis. This is a process through which specific locations in an organism’s genome can be altered in order to study the function of such locations, or proteins coded for by genes found in a location. There are many techniques available for site directed mutagenesis. (You have access to PCR ingredients, any primer you wish, laboratory equipment, Lambda phage, and the ability to alter Lambda genome, a commercially available...
When a eukaryotic organism (such as a human) gets a bacterial infection, it can be treated...
When a eukaryotic organism (such as a human) gets a bacterial infection, it can be treated with antibiotics. Antibiotics work by targeting prokaryotic cells without targeting eukaryotic cells. This is possible due to molecular differences between the two cell types that enable specific targeting of molecules in prokaryotic cells but not eukaryotic ones. The effects of different categories of antibiotics are listed below. For each one, explain how this would affect prokaryotic cells and why. Some antibiotics disable ribosome function....
1. if a lambda cl mutant infected e.coli , what a life cycle would be after...
1. if a lambda cl mutant infected e.coli , what a life cycle would be after infection? 2. lambda phage repressor protein control the prophage's lytic genes. true or false. explain your answer
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT