Question

Which one of the following labelled antibodies is NOT one of the most commonly used antibody in detection of target proteins in western blotting ?

Select one:

a. Gold-conjugated antibodies

b. Fluorophore-conjugated antibodies

c. SDS-conjugated antibodies

d. Biotinylated antibodies

e. Enzymes-conjugated antibodies

Answer 1

The gold nanoparticles are used for direct conjugation with thiol group-containing bio-molecules such as antibodies used in immuno-electron microscopy, immunohistochemistry and bio-assays.

Fluorescent labelled antibody uses a fluorescently labeled antibody to bind and illuminate a target antigen and seen under fluorescent microscope.

Th biotinylated antibodies are used for the detection of low-abundance proteins.

The enzyme conjugated antibody is used in Enzyme-linked immunosorbent assays (ELISAs), Blotting techniques, In situ hybridization, Cytochemistry and Histochemistry detection systems.

The correct answer is -

c. SDS-conjugated antibodies