Question

In the “Plasma and gill analyses” section of the methods section the authors wrote “Plasma Gh levels were measured by a radioimmunoassay (RIA) validated for Atlantic salmon by Björnsson et al. [37].” What would a low radioactive level suggest about Gh levels? (1 point)

In the “Plasma and gill analyses” section of the methods section the authors wrote “Plasma Gh levels were measured by a radioimmunoassay (RIA) validated for Atlantic salmon by Björnsson et al. [37].” What would a high radioactive level suggest about Gh levels? (1 point)

How do you know that your answer to questions 6 and 7 are correct? (1 point)

Answer 1

Radio immune assay or RIA is conducted to investigate the quantitative presence of a molecule of interest/antigen in a given sample. According to this assay, the intensity of radioactive signal represents direct correlation between the amount of antigen/molecule of interest present in the sample and the signal intensity on the collector. Thus, more is the signal intensity, more is the presence of analyte.

According to the inforamtion, the investigators intended to analyse the presene of Gh in the plasma using RIA.

Thus, high levels of radioactive signal would definitely infer high levels of Gh present in the sample. Similarly, low levels of radioactive signal would infer low levels of Gh present in the plasma sample.