Question

during sds page, if You see that the protein patterns (protein masses and protein intensity) differ between the different species and different tissues. Briefly explain why the patterns differ ?

and if you rated the quality of one or more lanes as poor, explain what the problem could have been. Are you able to store samples in the fridge and run them at a later date ?

Answer 1

SDS PAGE is a technique which is similar to electrophoresis which helps in the separation of proteins based on their mass.The protein will migrate towards the anode based on their masses.So different tissues will be having different protein mass and will show different types of patterns.Sometimes smearing can lead to a poor SDS PAGE,the main reason behind this is due to the unevenly poured acrylamide mixture or due to gross overloading of proteins.Yes,samples can be stored in the fridge.After taking from the fridge buffer and sds is added to the sample and then it is heated to a higher temperature to denature the proteins.