Distinguish between a BINDING assay and a FUNCTIONAL assay.
Binding assay- 1. It is an anlytic assay which relay on binding of ligand molecules to receptors or antibodies. It is of following type- Saturation assay, Competetitive assay, Kinetic assay.
Functional assay- 1. It is in vivo experiments that are designed to determine the involvement of protein in a cellular pathway.
2. It quantify the functioning of an active substance rather than its quantify just.
3. Main goal of functional analysis is to develop cell- based assay for pathogensis of Huntington's diseases. 4. It record a response to a given stimulation.
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