Question

A phage “plaque”: (select all that are true)

A. Indicates that the experiment is contaminated
B. Is formed when bacteria are lysed by phage growth on a section of plate
C. Shows that a mutation has occurred
D. Is expected when a recombinant DNA vector has been successfully created
E. Indicates that phage are dying by the millions
F. Normally originates from a single infection of a bacterium by a phage

Answer 1

A phage "plaque" is formed when bacteria are lysed by phage growth on a section of plate (option B) and Normally originates from a single infection of a bacterium by a phage (option F).

A bacteriophage will reduced turbidity of a agar plate in certain round of area is called plaque. It may be clear zone that without presence of bacteria growth. In the case of plaque, millions of bacteria are dying but viruses are growing rapidly using bacteria.

Plaque starts (infection) in a single bacterium and after that it effect surrounding bacteria and form a clear zone with dying of bacteria. Plaque is measured by PFU which means Plaque forming unit.

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