Question

1)If all other elements are wild type, a super repressor mutation (IS) in a bacterial cell...

1)If all other elements are wild type, a super repressor mutation (IS) in a bacterial cell that also has a constitutive operator mutation (Oc) results in

A) no transcription

B) transcription but no translation

C) no translation

D) constitutive transcription.

E) inducible transcription.

2) Which of the following is/are an example of a CIS-ACTING ELEMENT? (MAY BE MORE THAN ONE ANSWER)

A) b-galactosidase

B) Operator site

C) LacI repressor protein

D) Lactose

E) Permease

F) Lac promoter PXYZ

3) A promoter mutation (P-) results in: (MAY BE MORE THAN ONE ANSWER)

A) no transcription.

B) inducible transcription.

C) transcription but no translation.

D) no translation.

E) constitutive transcription.

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Homework Answers

Answer #1

1) a

No transcription

Is mutations are dominant , trans acting and uninducible . Oc mutations are dominant , cis acting and constitutive .

Transcription of structural gene will not occur because Is mutations are trans acting whereas Oc mutations are cis acting . Trans acting is dominant over cis acting.

2) f) lac promoter PXYZ

B) operator site

Cis acting element are those elements which are in close vicinity of the structural gene. Trans acting element are those elements which influence the structural gene irrespective of their position.

3) a) no transcription

D) no translation

P- mutations are cis acting , recessive and uninducible in nature.

In this mutation RNA polymerase cannot bind to the promoter as a result transcription will not occur . No transcription will result in no translation.

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