Question

QUESTION 44

1. Why does IPTG induce the lac operon?

   A.		Its an analog of lactose
   B,		It destroys lactose
   C.		it increases the amount of lactose in the cell
   D.		It reduces the concentration of cellular galactose

QUESTION 46

1. For CRISPR-Cas to work on different genes, you have to alter the sequence of _____________ to provide specificity for where it cuts the DNA.

   A.		single guide RNA
   B.		the host gene
   C.		Caspase domain
   D.		protospacer adjacent motif

QUESTION 48

1. An RNA with a 5'-cap is degraded more quickly than one lacking a 5'-cap.

   A. True

   B. False  

QUESTION 49

1. If a mutation was made in an operon where the repressor could NEVER bind to the operator, what would you predict about expression of the genes in the operon.

   	A.	It would have no impact on expression
   	B.	The genes would all be expressed.
   	C.	Sucrose levels would increase
   	D.	None of the genes would be expressed

QUESTION 50

1. A gene encoding what enzyme must be present in a transposon in order for it to function?

   A.		Direct Repeats
   B.		RNA polymerase
   C.		Transposase
   D.		Inverted Repeats

Answer 1

44. Ans: *A* IPTG is Isopropyl beta D-1 thiogalactopyranoside which is molecular mimicry of allolactose and there by triggers transcrption of la operon.

46.Answer: single guide RNA

In crispr cas 9 work , cas nuclease is complexed with synthetic guide RNA into the cell. The cells genome can be cut into specific location.

48.False because 5 'caping provides protection from degradation and also to provide ribosome binding during translation.

49.Answer :Genes would all be expressed., Because when repressor no longer bind to operator,RNA polymerase will bind to operator to express all the genes.

50.Answer Transposase which self encoded recombinase with the help of this enzyme transposon worked.