Question

In the course of the Drosophila melanogaster genome project, the following genomic DNA sequences were obtained. Try to assemble the sequences into a single contig.

5? TTCCAGAACCGGCGAATGAAGCTGAAGAAG 3?

5? GAGCGGCAGATCAAGATCTGGTTCCAGAAC 3?

5? TGATCTGCCGCTCCGTCAGGCATAGCGCGT 3?

5? GGAGAATCGAGATGGCGCACGCGCTATGCC 3?

5? GGAGAATCGAGATGGCGGACGCGCTATGCC 3?

5? CCATCTCGATTCTCCGTCTGCGGGTCAGAT 3?

Using the assembled sequence, perform a blastn search using the “nucleotide collection (nr/nt)” database. Does the search produce sequences similar to your assembled sequence, and if so, what are they? Can you tell if your sequence is transcribed, and if it represents protein-coding sequence? Perform a tblastx search, first choosing the “nucleotide collection (nr/nt)” database and then limiting the search to human sequences by typing Homo sapiens in the organism box. Are homologous sequences found in the human genome? Annotate the assembled sequence.

https://blast.ncbi.nlm.nih.gov/Blast.cgi

Answer 1

Ans) The complete sequenc is :-

5'TTCCAGAACCGGCGAATGAAGCTGAAGAAGGAGCGGCAGATCAAGATCTGGTTCCAGAACTGATCTGCCGCTCCGTCAGGCATAGCGCGTGGAGAATCGAGATGGCGCACGCGCTATGCCGGAGAATCGAGATGGCGGACGCGCTATGCCCCATCTCGATTCTCCGTCTGCGGGTCAGAT 3'

Ans) The Blast search denote sequence similar with various other sequence similarity and "Drosophila melanogaster strain rover (forR) chromosome 3R" is one of them.

Ans) Performing tblastx search, the homologous sequence is similar with the Homo sapiens.That sequence similarity is "Homo sapiens genomic DNA, chromosome 20, clone:CTB-271B20, complete sequence", "Homo sapiens chromosome 3 clone RP11-447D11, complete sequence"