Question

On agar plate does each discrete colony represent the growth of one cell ? explain your answer. Why can a single colony on a plate be used to start a pure culture ?

Answer 1

Yes,each discrete colony represent the growth of one cell because the mixture was being spread out over the agar plate.

A single colony on agar plate can also be used to start a pure culture because that only arose from single cell and divides to create single species.

Additionally mixture is added to one area of dish/plate and the mixture is then spread over the remaining three areas of plate and then individual cells are spread out over the dish and give rise to separate colonies.