If mannitol is fermented to produce acid end products, the pH of the medium will drop. A pH indicator in the medium is responsible to change the color to indicate acid production. The positive test consists of a color change from red to yellow, determining a pH change to acidic. The negative test indicates no color change.
A positive result of mannitol fermentation test from Pseudomonas aeruginosa will produce a yellow halo around the bacterial colony. This is the indication of acid production from the breakdowm of mannitol.
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