Question

2. For each sample you test in lab this week, you will set two PCR reactions....

2. For each sample you test in lab this week, you will set two PCR reactions. One of these reactions, labeled “Plant”, amplifies regions of DNA found in all plants. What is the purpose of this reaction? What does it tell you about your experiment?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

PCR or Polymerase Chain Reaction, is technique used for making several copies of a gene of interest. using thermostable enzyme Taq polymerase These copies can the be used for various purposes like cloning, analysis by techniques like gel electrophoresis or for determining nucleotide sequence in a gene. It can also be used to identify any genetic disorder even in the fetus or for determining the genetic makeup of a virus.

It will tell that the reaction labeled plant, contains DNA to be amplified as well as other required things like specific primers and Taq polymerase. The reaction is not a negative control (doesnot contains DNA to be amplified but all other components of PCR) but either a test or a postive control (contains known DNA with all other required components). It also indicates that the experiment is working properly.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is...
As a reminder, PCR is the technique used to replicate DNA in the lab. PCR is based on the way DNA is replicated in cells. During PCR template DNA plus other ingredients are placed in a test tube and the solution goes through repeated cycles of heating and cooling. You can find more information about PCR here. The 'ingredients' of a basic PCR reaction are: DNA template, primer, nucleotides, DNA polymerase, and any salts required for polymerase function (buffer). No...
Suppose you are a student performing research in a molecular genetics lab. Your project involves PCR...
Suppose you are a student performing research in a molecular genetics lab. Your project involves PCR amplification of your own Hemoglobin gene. One day, you decide to check your Huntington’s disease risk by amplifying your own HTT gene. (PSA: This is just a test question! Genetic counselors recommend consulting a healthcare provider for any DNA testing.) What component must be changed in the PCR to check for the risk of Huntington’s disease? a) The DNA template b)The DNA polymerase c)...
1. Suppose you start graduate school to be a plant physiologist and your first lab rotation...
1. Suppose you start graduate school to be a plant physiologist and your first lab rotation is in a lab that studies gibberellic acid (GA) in peas. Unfortunately one of the graduate students in the lab labeled the packets of seeds for two mutants with dry-erase marker instead of permanent marker and the labels rubbed off. One mutant had a defective GA biosynthesis gene and the other had a defective GA receptor gene. Your boss suggested isolating DNA and doing...
Purpose: In this laboratory you will carry out a quantitative real-time PCR (QPCR), using SYBR Green,...
Purpose: In this laboratory you will carry out a quantitative real-time PCR (QPCR), using SYBR Green, on the samples that you purified last laboratory session. QPCR serves several purposes in a forensic laboratory and is included in the sample processing workflow for all/most DNA samples. QPCR ultimately measures (either in absolute numbers or relatively) the amount of DNA in a sample and the presence of any inhibitors (either partial or complete). This allows the forensic scientist to determine if more...
Class, as you will see in your lab from this week there are a couple of...
Class, as you will see in your lab from this week there are a couple of different mechanisms that take place - two that we can start with mechanical digestion and chemical digestion. What can you tell me about these two and where do they occur?
Procedure I. Reverse Transcription. For each of your RNA samples set up the following reaction: *Remember...
Procedure I. Reverse Transcription. For each of your RNA samples set up the following reaction: *Remember to use proper RNA handling technique! 1. In a 0.2 ml size RNAse-Free tube add the following: • 0.5 µg RNA (no more than 6 µl) You can add as little as 0.1 µg of RNA if your concentration is low • 2 µl dT primer • Nuclease Free Water to final volume of 8 µl * Before you start write the exact volume...
Experiment 2: Restriction Enzymes (Pop-It Bead Lab) Post-Lab Questions How can DNA be fragmented into very...
Experiment 2: Restriction Enzymes (Pop-It Bead Lab) Post-Lab Questions How can DNA be fragmented into very specific sections? Where do restriction enzymes come from? What is their function in nature? How do molecules of varying sizes separate in electrophoresis? What is the purpose of the gel? What about the electricity? Investigate one way in which electrophoresis is used in medicine today. Write one or two paragraphs to explain your findings.
Lab: synthesis reaction of anthranilic acid and acetic anhydride, which produces N-acetylanthranilic acid. fluorescence showed the...
Lab: synthesis reaction of anthranilic acid and acetic anhydride, which produces N-acetylanthranilic acid. fluorescence showed the crystals to be all green/yellow, what does the color tell you? how can you tell you seperated every thing in this lab? is there only one product formed with this reaction or two?
Imagine that you are part of a lab group planning a metagenomic survey of microbial life...
Imagine that you are part of a lab group planning a metagenomic survey of microbial life living inside the liquid found in pitcher plants (carnivorous plants) found in bogs in Michigan. You're asked to design an experiment using only filters that will enrich for three samples: A) Eukaryotic cells; B) Archaeal and Bacterial cells; and C) Viruses. A) Describe a filtering protocol (be specific about filter pore size) that will successfully generate the three enriched fractions described above. You may...
Experiment 1: Observe the Reactions Between Halide and Calcium Ions Take four test tubes from the...
Experiment 1: Observe the Reactions Between Halide and Calcium Ions Take four test tubes from the Containers shelf and place them on the workbench. Fill each test tube with a different halide solution from the Materials shelf, as directed below. You may want to label the test tubes by double-clicking on each test tube and renaming it. Test Tube # Halide Solution 1 10.5 mL 0.1 M sodium chloride 2 10.5 mL 0.1 M sodium fluoride 3 10.5 mL 0.1...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT