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2. For each sample you test in lab this week, you will set two PCR reactions....

2. For each sample you test in lab this week, you will set two PCR reactions. One of these reactions, labeled “Plant”, amplifies regions of DNA found in all plants. What is the purpose of this reaction? What does it tell you about your experiment?

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PCR or Polymerase Chain Reaction, is technique used for making several copies of a gene of interest. using thermostable enzyme Taq polymerase These copies can the be used for various purposes like cloning, analysis by techniques like gel electrophoresis or for determining nucleotide sequence in a gene. It can also be used to identify any genetic disorder even in the fetus or for determining the genetic makeup of a virus.

It will tell that the reaction labeled plant, contains DNA to be amplified as well as other required things like specific primers and Taq polymerase. The reaction is not a negative control (doesnot contains DNA to be amplified but all other components of PCR) but either a test or a postive control (contains known DNA with all other required components). It also indicates that the experiment is working properly.

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