Question

1. What amount of agarose (g) and TAE buffer (mL) would you need to make a 1% gel?

Answer 1

Agarose gel electrophoresis is a technique which is used for separation of DNA and proteins from their mixture. For this technique, Agarose gel buffer is used. Agarose gel buffer is the mixture of Agarose into the TAE (Tris-acetate-EDTA) buffer.

Here, for making 1% 100ml of the agarose gel buffer, 1 gram of the agarose is mixed with 100 ml of the TAE buffer.

Similarly, for making 1% 1000 ml (1 litre) of the agarose gel buffer we need to mix 10 gram of agarose into the 1000ml of the TAE buffer.

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