Question

How is senescence measured?

Answer 1

There are several assays made by researchers to find senescence. One of the best simplified and characterized method to measure senescence in vitro and vivo is the  β-galactosidase (β-Gal) assay, which measures β-Gal activity expressed by senescent cells that can be detectable at PH 6.0 by immunohistochemistry. The colorimetric substrates for β-Gal , 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside this known as x- gal has long been used to detect metabolic activity in two cells in vitro.