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An isolated soluble peripheral enzyme can be inhibited for a short time and then recovered to...

An isolated soluble peripheral enzyme can be inhibited for a short time and then recovered to full activity by either (a) adding a chaotropic agent like urea, OR (b) freeze the protein. Which one?

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Answer #1

Correct option (b)

Chaotropic agents disturbs the structure of protien and denatures the protien. A chaotropic agent is a molecule that disturbs non-covalent bonds .This increases the entropy of a substance, which correlates with the increase in kinetic energy of molecules. This results ordered structures into disordered structure. Due to this protein tertiary structure is destroyed.It reduces enzyme activity, or prevent it from working.Low temperature preserves the enzymes in their inactive state. When enzyme gets back to optimum temperature,enzyme get activated with its full activity.So An isolated soluble peripheral enzyme can be inhibited for a short time and then recovered to full activity by freeze the protien.

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