Question

How will I be able to structure/form an experiment to tell whether or not my cells will express a certain transmembrane protein?

Answer 1

Answer:- Membrane protein crystallograpy has the potential to greatly aid our understanding of membrane protein biology. Yet, membrane protein crystals remain challenging to produce. Although robust methods for the expression and purification of membrane protein continue to be developed,the detergent component of membrane protein samples are equally important to crystallization efforts. All of these techniques use small volume and have potential applications in crystallograpy. The application of these techniques in crystallization pre-screening, detergent concentration modification, and detergent exchange experiment is demonstrated. It has been observed that the concentration of the detergent in the membrane in protein samples can be just as important as the protein concentration when attempting to reproduce crystallization lead conditions.