Question

How does the isolation of a mutant yeast that
accumulates vesicles provide information on the
process of protein trafficking?

Answer 1

Vesicles are small enclosed sacs which remain engaged in storing and transporting of substances from one location to other. It transport proteins from its site of synthesis to the site of their functions. Vesicles is consist of two lipid bilayers in which the specific proteins are stored and remain separated from rest of the cytoplasm. The correct transport of proteins through vesicles process requires signalling molecules. Transport vesicles often found to transport proteins from endoplasmic reticulum to Golgi bodies. So if we found a yeast which is mutant and accumulates vesicles, then we can trace the pathway by which the specific protein are transported. It is like knock out mice where specific gene elimination helps us to find out its specific function in the cell.