Question

Please answer questions 1-4 thank you:)

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1. Why does infection with human immunodeficiency virus, if left untreated, ultimately lead to a loss of both humoral and cellular immunity?

2. Compare and contrast RT-PCR and ELISA for detecting a virus infection. What is each one detecting and how? Describe a relative pro and con for each.

3. Explain why autoimmune antibody production can result in both type II and type III hypersensitivity.

4. Explain these two possible mechanisms for generating autoreactive helper T cells: release of sequestered antigen and molecular mimicry.  

Answer 1

1. Human immunodeficiency virus or HIV is a virus that causes the serious condition called Aquired Immuno deficiency Syndrome or otherwise called as AIDS. If this virus is left untreated, it inturn leads to loss of humoral and cellular immunity. There are some aspects of immunity that is mediated by macromolecules that are usually found in extracellular fluids like humoral immunity. CD4+ T-cells are central mediators/facilitators of immune response in humans.They are respnsible for coordinating cellular and humoral immune responses in our body against infections. HIV particularly destroys and infects. CD4+T cells for their own benefits.

2.

RT- PCR	ELISA
Low reagent costs	Moderate reagent costs
Detection limit is 1-10 ng/μL	Detection limit is 0.01/μL