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74. Which of the following is the best second-generation sequencing method to examine the whole human...

74. Which of the following is the best second-generation sequencing method to examine the whole human genome?

A. Ion semiconductor sequencing

B. Sanger Sequencing

C. Pyrosequencing

D. Sequencing by synthesis

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Answer #1

Answere : The correct option is option (A) i.e. Ion semiconductor sequencing.

Explanation : Ion semiconductor sequencing  (Ion Torrent Sequencing) is the best second-generation sequencing method to examine the whole human genome.

This method of DNA sequencing is based on the hydrogen ions detection. Hydrogen ions are released during DNA polymerization. It utilizes release of hydrogen ions during sequencing reaction to detect sequencing of a cluster.

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