Question

Poor cell culture technique could result in damage to the cell membrane during the transfer of...

Poor cell culture technique could result in damage to the cell membrane during the transfer of cells. Why would this type of poor technique be particularly concerning if one was conducting an LDH assay?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Answer:

LDH (Lactate dehydrogenase) assay is the analysis of cell viability. The cytotoxicity is determined by the activity of cytoplasmic enzymes which is released by damaged cells. The damaged cell plays an important role in LDH assay. When the Plasma membrane is damaged LDH is released into cell culture and it is quantified by the NADH produced during the conversion of lactate to Pyruvate.

It also reflects the reduction of Yellow Tetrazolium salt, INT, by NADH into a red, water-soluble formazan. The amount of formazan is directly reflected the amount of LDH in culture and LDH states the no of damaged cells.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Question 8. A transmembrane protein uses a β barrel structure to span the cell membrane. How...
Question 8. A transmembrane protein uses a β barrel structure to span the cell membrane. How can amino acid mutations in a protein within this β barrel structure affect interactions with the membrane and why? (Up to 50 words) Question 9. Haemoglobin is a protein that carries oxygen in our red blood cells. Sickle cell anaemia is a genetic disease in which Glu at position 6 of haemoglobin is mutated to a Val, rendering haemoglobin non-functional. (i) What type of...
The scientific method is best distinguished from earlier forms of knowledge by Accurate measurements              b. inductive...
The scientific method is best distinguished from earlier forms of knowledge by Accurate measurements              b. inductive reasoning           c. deductive reasoning d. publishing of results                 e. experimentation Which one word best describes a tentative explanation of the data? a. control        b. deduction    c. theory          d. placebo       e. hypothesis An explanation which has been widely tested, which is not found to be false, and which explains a large group of observations is called                      a. quantitative analysis           b. a theory Qualitative...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old man of Scandinavian descent was rushed to the Emergency Room of your local hospital after a family member discovered him unconscious in his home. The woman who dialed “911” told the dispatcher that the man, her brother, was the local librarian of the past 10 years and had no spouse or children. She reported that they had spoken the day before, and he had...
Part III – Something's Not Right “It’s good to have you home, honey. I missed you....
Part III – Something's Not Right “It’s good to have you home, honey. I missed you. How was the flight?” Stacey had come to the airport to pick Frank up and she leaned over to kiss him as he climbed into the car with his luggage. “How were the meetings? You look tired,” she added. “The past week was intense and I am exhausted. I thought I would manage some R & R during the trip, but no such luck....
What role could the governance of ethics have played if it had been in existence in...
What role could the governance of ethics have played if it had been in existence in the organization? Assess the leadership of Enron from an ethical perspective. THE FALL OF ENRON: A STAKEHOLDER FAILURE Once upon a time, there was a gleaming headquarters office tower in Houston, with a giant tilted "£"' in front, slowly revolving in the Texas sun. The Enron Corporation, which once ranked among the top Fortune 500 companies, collapsed in 2001 under a mountain of debt...
52. BMR stands for basic memory retention basal metabolic rate bregmatic myofascial release none of these...
52. BMR stands for basic memory retention basal metabolic rate bregmatic myofascial release none of these 53. The primary (not initial) fuel during endurance exercise is liver glycogen muscle glycogen fatty acids none of these 54. The human body consists of chemicals cells tissues none of these all of these 55. The main purpose of the digestive system is the breakdown of foods and waste elimination to supply oxygen and eliminate carbon dioxide reproduction and maintenance of sexual characteristics none...
When is a hypothesis considered scientific? a. when it is based on something other than observation...
When is a hypothesis considered scientific? a. when it is based on something other than observation b. when it can be tested and is refutable c. when it relies on anecdotal evidence d. when it relies on mystical explanations e. All hypotheses are considered scientific until experiments determine otherwise. 3. Of the following, which is the earliest step in the scientific process? a. generating a hypothesis b. analyzing data c. conducting an experiment d. drawing a conclusion e. developing a...
Question: A Nurse Practitioner educator who coordinates the staff education on an oncology unit is conducting...
Question: A Nurse Practitioner educator who coordinates the staff education on an oncology unit is conducting an inservice on … therapies. What potential benefit of … therapies should the nurse highlight in this education session? NURS 6521N 55 Question: Laboratory testing has confirmed that a patient has chloroquine-resistant malaria and the patient’s physician has … quinine along with an adjunctive drug. The nurse should question the physician’s order if the patient has a history of Question: When planning care for...
2. SECURING THE WORKFORCE Diversity management in X-tech, a Japanese organisation This case is intended to...
2. SECURING THE WORKFORCE Diversity management in X-tech, a Japanese organisation This case is intended to be used as a basis for class discussion rather than as an illustration of the effective or ineffective handling of an administrative situation. The name of the company is disguised. INTRODUCTION In light of demographic concerns, in 2012, the Japanese government initiated an effort to change the work environment in order to secure the workforce of the future. Japan is world renowned for its...
Discuss ethical issues that can be identified in this case and the mode of managing ethics...
Discuss ethical issues that can be identified in this case and the mode of managing ethics Enron finds itself in this case. How would you describe the ethical culture and levels of trust at Enron? Provide reasons for your assessment. THE FALL OF ENRON: A STAKEHOLDER FAILURE Once upon a time, there was a gleaming headquarters office tower in Houston, with a giant tilted "£"' in front, slowly revolving in the Texas sun. The Enron Corporation, which once ranked among...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT