NAD absorbs light at 258 with a molar extinction coefficient of 18,000. NADH absorbs light at 258 with a molar extinction coefficient of 15,000 and also absorbs light at 340 with a molar extinction coefficient of 6,220. You have prepared a mixed solution of NAD and NADH of unknown concentration. The absorbance of the mixture is 1.3 at 258 nm and 0.32 at 340 nm. What is the concentration of NAD? NADH? (express your answers in microM)
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