Question

NAD absorbs light at 258 with a molar extinction coefficient of 18,000. NADH absorbs light at...

NAD absorbs light at 258 with a molar extinction coefficient of 18,000. NADH absorbs light at 258 with a molar extinction coefficient of 15,000 and also absorbs light at 340 with a molar extinction coefficient of 6,220. You have prepared a mixed solution of NAD and NADH of unknown concentration. The absorbance of the mixture is 1.3 at 258 nm and 0.32 at 340 nm. What is the concentration of NAD? NADH? (express your answers in microM)

Homework Answers

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
The protein metmyoglobin and imidazole form a complex in solution. The molar extinction coefficient for metmyoglobin...
The protein metmyoglobin and imidazole form a complex in solution. The molar extinction coefficient for metmyoglobin is 0.942L/mol cm at 500 nm . The molar extinction coefficient for complex is 0.688L/mol cm at 500nm Equilibrium is established in a solution which has an initial concentration of metmyglobin equal to a 0.59M. The soluton reaches equilibrium and demonstrates an absorbance of 0.435 at 500 nm. Assuming the reaction is metmyglobin ----------> complex What is the value of K, the equilibrium constant...
The molar extinction coefficient of reduced NADH (nicotinamide adenine dinucleotide phosphophate) at 340nm is 6220 L/mole·cm....
The molar extinction coefficient of reduced NADH (nicotinamide adenine dinucleotide phosphophate) at 340nm is 6220 L/mole·cm. 11ml of solution containing 0.8 micromoles of NADH were placed in a cuvette of 1.6cm light path. Calculate the percentage light transmission of this sample at 340nm.
The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist...
The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.161. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express the answer in micromolar (notice micro means 10-6) Concentration of Blue Dye(M) Absorbance @...
The extinction coefficient for FD&C Blue Dye is #1 is 138,500 cm-L/mol at 630nm. A chemist...
The extinction coefficient for FD&C Blue Dye is #1 is 138,500 cm-L/mol at 630nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.309. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution.