1) The following figure represents experimental results from study designed after the Beadle and Tatum experiment.
Assume the mutants are homozygous for recessive alleles causing their phenotypes.
Nutrient R | Nutrient T | Nutrient X | Nutrient Z | |
Mutant 1 | Yes | No | Yes | Yes |
Mutant 2 | No | No | Yes | Yes |
Mutant 3 | Yes | Yes | Yes | Yes |
Mutant 4 | Yes | Yes | Yes | Yes |
Mutant 5 | No | No | Yes | No |
A) Construct a biochemical pathway relating nutrients and genes based on the data. Show the order of the synthesis of the nutrients, the enzymes (name the enzymes A, B, C, and D), and show where each mutant has a defective enzyme.
Enzymes: A B C D Precursor ----M1----> Nut1 ----M2----> Nut2 ----M3---> Nut3 ----m4---> Nut4
M1: ["Mutant 1", "Mutant 2", "Mutant 3", "Mutant 4", "Mutant 5", "Mutants 1 and 2", "Mutants 3 and 4", "Mutants 1 and 5"]
M2: ["Mutant 1", "Mutant 2", "Mutant 3", "Mutant 4", "Mutant 5", "Mutants 1 and 2", "Mutants 3 and 4", "Mutants 1 and 5"]
M3: ["Mutant 1", "Mutant 2", "Mutant 3", "Mutant 4", "Mutant 5", "Mutants 1 and 2", "Mutants 3 and 4", "Mutants 1 and 5"]
M4: ["Mutant 1", "Mutant 2", "Mutant 3", "Mutant 4", "Mutant 5", "Mutants 1 and 2", "Mutants 3 and 4", "Mutants 1 and 5"]
Nut1: ["Nutrient R", "Nutrient T", "Nutrient X", "Nutrient Z"]
Nut2: ["Nutrient R", "Nutrient T", "Nutrient X", "Nutrient Z"]
Nut3: ["Nutrient R", "Nutrient T", "Nutrient X", "Nutrient Z"]
Nut4: ["Nutrient R", "Nutrient T", "Nutrient X", "Nutrient Z"]
B) What is the genotype of Mutant 2?
["AABBCCDD", "aaBBccdd", "AaBbCcDd", "AAbbCCDD", "AABBccDD", "aabbCCdd", "AABbCcDD"]
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