Which of the following statements about RNA synthesis in prokaryotes is correct?
A. RNA is synthesised in one step in prokaryotes.
B. The core enzyme of RNA polymerase recognises the transcription initiation site when it binds to the promoter region.
C. The Pribnow box is the -35 consensus nucleotide sequence 35 bases downstream from the transcription start site.
D. By biochemical convention, regions to the left of the transcription start sites are known as "downstream".
E. The nucleotide sequence of the promoter determines its strength.
Option E. The nucleotide sequence of the promoter determines its strength is the answer. The strength of promoter depends on nucleotide and state of the cell.
RNA synthesis is done in three steps in prokaryotes and they are simple . The signal factor recognises transcription initiation site and core factor can continue transcription after. The prinbow box is 35 bases upstream from the transcription start site. By convention, region to the left of transcription site are upstream.
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