Question

Let’s suppose you have the freedom to choose any of the expression systems below. Which one would be your first choice to manufacture your product – considering only viral safety this time (safety means: absence of undesirable viruses which may be infectious and/or pathogenic for humans)

a. CHO

b. PERC.6

c. E.coli

d. Transgenic animal

e. Transgenic plant

Answer 1

I will choose the E.Coli because :

E. coli is the name of a type of bacteria that lives in your intestines and in the intestines of animals. Although most types of E. coli are harmless, some types can make you sick. Since we are only concerned about the virus, all other options contain virus.E. Coli mostly are virus free since they are not hosting to viruses.

The expression of proteins in E. coli is the easiest, quickest and cheapest method. There are many commercial and non-commercial expression vectors available with different N- and C-terminal tags and many different strains which are optimized for special applications (for local users: see strain database)