Question

1. RFLPs, used in the search for the Huntington's Disease gene, can be used to identify point mutations through which of the following mechanisms?

Group of answer choices

a. The mutation can occur in a restriction site, causing a change in the cutting pattern for restriction enzymes.

b. The mutation can be silent in the coding for protein resulting in a different amino acid being inserted.

c. The mutation can occur outside of a restriction site causing a change in the cutting pattern for restriction enzymes.

d. The mutation can occur causing a shortening of the mRNA that is then compared to wild type.

2.

SNPs are characterized by being ________.

Group of answer choices

a.highly heterogeneous with variable amino acid substitutions

b. highly uniform in the population with variable numbers of tandem repeats

c. variable in the population with variable base sequences

d. homogeneous with various nucleotides transcribed repeatedly

3. The genomes of several hundred to several thousand diseased individuals are subject to individual genome sequencing as well as several thousand of non-diseased individuals. The genomes are then compared to determine if there are patterns or associations between certain genes and the disease state. This does not mean a carrier of the correlated gene will develop the disease, just that there is a risk factor. This was done with age-related macular degeneration and is called:

Group of answer choices

a. Genome Wide Association Studies (GWAS)

b. HapMap

c. RFLP

d. QTL

Answer 1

1. Answer: Option A is correct
Explanation:
RFLP = Restriction fragment length polymorphism
It utilizes an SNP that creates are abolishes a restriction site in the mutant.
Due to this, the restriction digestion pattern would be different between the WT and the mutant.

2. Answer: Option C is correct
Explanation:
SNP = Single nucleotide polymorphism
They are variable in base sequence in a given population.

3. Answer: Option A is correct
Explanation:
GWAS = Genome-wide association studies
In GWAS, genomes from several individuals (Both WT and mutant or form different geographical regions) are sequenced and analyzed for the presence of variations and their association with a particular phenotype/disorder.