Question

What is the function of the RNase H domain in HIV-1 RT? I know RNases breakdown...

What is the function of the RNase H domain in HIV-1 RT? I know RNases breakdown RNA, but what is it breaking down? Because from what I know R.T does not have proofreading ability. What does this RNase H domain do when replicating DS Dna from SS RNA?

Homework Answers

Answer #1

when replicating DS DNA from SS RNS, the RNase H domain cleaves the RNA molecules at RNA / DNA hybrid juction. it breaking down the RNA molecules hence the DS DNA can be formed. generally RNase H claved (+) RNA strand and also it create PPT primer (polypurinated track) at 5’ end. The PPT site is different as it can not cleaved by RNase H. this intact PPT site later on work as aprime sire for +DNA strand synthesis.

The RNase H breaks the RNA molecules in oligonucleotides. after DNA is formed from RNA (in case of RT virus), the RNA sequence is no longer useful and it is destroyed/ break down by RNase H.

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