when replicating DS DNA from SS RNS, the RNase H domain cleaves the RNA molecules at RNA / DNA hybrid juction. it breaking down the RNA molecules hence the DS DNA can be formed. generally RNase H claved (+) RNA strand and also it create PPT primer (polypurinated track) at 5’ end. The PPT site is different as it can not cleaved by RNase H. this intact PPT site later on work as aprime sire for +DNA strand synthesis.
The RNase H breaks the RNA molecules in oligonucleotides. after DNA is formed from RNA (in case of RT virus), the RNA sequence is no longer useful and it is destroyed/ break down by RNase H.
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