Question

I​n CRISPR gene editing match the molecule with its function

cas9 nuclease--

sgDNA--

donor DNA--

• A. target mRNAs for degradation

• B. cleaves sgRNA for maturation

• C. produces double stranded breaks in target

• D. contains an edited sequence that will be used for homology dependent repair

• E. identify target DNA sequences for cleavage

I think sgRNA=E and donor DNA=D, but can someone check that and help me with cas9 nuclease?

Answer 1

CRISPR GENE is usually used to edit or repair the gene so that they can alter the function of genome.

it contain mainly three parts

1)sgDNA :OPTION E-identify target DNA sequences for cleavage

the function of this DNA ia to guide the Ca9 nuclease enzyme to the DNA sequence where the alteration is to be done.

2)OPTION C -produces double stranded breaks in target

Cas 9 nuclease: it is the endonuclease enzyme that help to cut the double strand in the target place that is shown by the sgDNA.

This enzyme cleaves the end of DNA, after that,there can be addition or deletion of DNA segement occur , this produce the mutation of the gene.

3)donor DNA- contains an edited sequence that will be used for homology dependent repair