Question

all about eosin methylene blue agar

Answer 1

Eosin methylene blue (EMB).

It's also called the levine formulation.

It's strain that is used for the mainly gram nagative bacteria. EMB contain the dye that is harmful to or toxic to the gram positive bacteria.

Its is formed by the mixture of the two strain eosin and methylene blue dye in the ratio of the 6.1

EMB formed by many ingredients like the lactose,peptone,dipotassium phosphate, esoin dye, methylene blue dye and agar.

It's differentiating the lactose fermentation bacteria and non fermented bacteria by the colour indicator that's why it's called the distinguish indicator.

Those bacteria do fermentation lactose show the nucleated colonies with dark center.

Those bacteria do rapid lactose fermentation produce acid so that pH is low and show the colour purple black

Those do not lactose fermented do not show colour because increased pH because of the protein breakdown.