Question

Answer all questions in sequence, using the oligopeptide.

5’-AACGATGCCATCAGAGCCCAGGACGTGATTTAA-3’

3’-TTGCTACGGTAGTCTCGGGTCCTGCACTAAATT-5’

1) Which is the template strand?

2) Write each functional strand and underline ea functional codon. Make sure to write 5’/3’ position.

3) Using one theee letter abrev for amino acid, what is the residue sequence? Make sure to label the ends.

Answer 1

Please find the answers below:

Answer 1: The template strand of the DNA is the one which upon which the RNA polymerase binds for generation of mRNA. This template strand has the orientation of 3' to 5'. Thus, the lower strand of the DNA represents the template strand.

Answer 2: The upper strand is the coding or non-template strand. The lower strand is the non-coding or the template strand. Transcription initiation takes place from the codon ATG and terminates at TGA codon of the coding strand. The final sequence of the amino acid would be given by:

5' AUG CCA TCA GAG CCC AGG ACG 3'

Answer 3: The final sequence of the amino acids encoded would be given by:

5' Met-Pro-Ser-Glu-Pro-Arg-Thr 3'